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Updated: Aug 19, 2026
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
Published on: February 15, 2021
[Experimental study on the myogenic differentiation of marrow mesenchymal stem cells in the local muscle tissues]
Jing Wang1, Chengji Luo, Hui Xu
1Institute of Combined Injury of PLA, Third Military Medical University, Chongqing 400038, P R China. wangj5768@yahoo.com.cn
Objective:
To investigate the myogenic differentiation of mesenchymal stem cells (MSCs) after being transplanted into the local muscle tissues.
Methods:
The serious muscle-injured model was established by the way of radiation injury, incising, and freezing injury in 36 mice. Purified MSCs derived from bone marrow of male mouse and MSCs induced by 5-azacytidine (5-Aza-CR) were transplanted into the local of normal muscle tissues and injured muscle tissues of female mouse. The quantity of MSCs and the myogenic differentiation of implanted MSCs were detected by the method of double labeling, which included fluorescence in situ DNA hybridization (FISH) and immunohistochemistry on the 1st, 3rd, 6th, 9th, 12th, and 15th day after transplantation.
Results:
The quantity of implanted MSCs decreased as time passed. MSCs' differentiation into myoblasts and positive expression of desmin were observed on the 15th day in purified MSCs group and on the 6th day in induced MSCs groups.
Conclusion:
MSCs could differentiate into myoblasts after being implanted into the local of muscle tissues. The differentiation occurs earlier in the induced MSCs group than that in purified MSCs group.
