Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Clone-array pooled shotgun mapping and sequencing: design and analysis of experiments.

Miklós Csürös1, Bingshan Li, Aleksandar Milosavljevic

  • 1Département d'informatique et de recherche opérationnelle, Université de Montréal, CP 6128 succ. Centre-Ville, Montréal, Québec H3C 3J7, Canada. csuros@iro.umontreal.ca

Genome Informatics. International Conference on Genome Informatics
|February 12, 2005
PubMed
Summary

This study enhances Clone-Array Pooled Shotgun Sequencing (CAPSS) for whole genome assembly and introduces Clone-Array Pooled Shotgun Mapping (CAPS-MAP) for BAC ordering. Both methods utilize pooled subclone libraries for efficient genomic analysis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancements in extracellular vesicle research.

Extracellular vesicle·2026
Same author

Integration of lung tissue proteomics and genome-wide association data to identify lung cancer susceptibility proteins and potential drug targets.

medRxiv : the preprint server for health sciences·2026
Same author

Real-time, automated, standardized, and transparent analysis of microfluidic nanoparticle data with RPS<sub>PASS</sub>.

bioRxiv : the preprint server for biology·2026
Same author

Effects of forest bathing versus indoor gym exercise on exercise-related boredom: a randomized crossover trial with repeated measures.

Frontiers in psychology·2026
Same author

Multi-ancestry transcriptome-wide association studies uncover insights into breast cancer genetics and biology.

Nature communications·2026
Same author

Integrating genetically predicted transcriptomic signatures with longitudinal real-world data enables scalable drug repurposing for Alzheimer's disease.

Research square·2026

Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Whole genome sequencing and physical mapping are crucial for understanding genome structure and function.
  • Current methods often involve complex and time-consuming steps for assembling and ordering large genomic clones.

Purpose of the Study:

  • To improve existing pooled shotgun sequencing methods for genome assembly.
  • To develop a novel method for physical mapping of bacterial artificial chromosomes (BACs).
  • To enable efficient and accurate large-scale genomic analyses using pooled clone libraries.

Main Methods:

  • Scrutinizing and refining the Clone-Array Pooled Shotgun Sequencing (CAPSS) method.
  • Introducing Clone-Array Pooled Shotgun Mapping (CAPS-MAP) for BAC ordering.

Related Experiment Videos

  • Constructing subclone libraries from pooled genomic BAC clones for both methods.

    • Main Results:

    • Demonstrated improvements in the CAPSS method for BAC-linked genome assembly.
    • Successfully developed and validated the CAPS-MAP method for physical ordering of BACs.
    • Showcased the utility of pooled subclone libraries in both sequencing and mapping.

    Conclusions:

    • The enhanced CAPSS and novel CAPS-MAP methods offer efficient approaches for whole genome sequencing and physical mapping.
    • These pooled library-based strategies facilitate large-scale genomic research.
    • The developed methods contribute to advancing genome assembly and structural analysis techniques.