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Updated: Aug 19, 2026

Isolation of High Quality Murine Atrial and Ventricular Myocytes for Simultaneous Measurements of Ca2+ Transients and L-Type Calcium Current
Published on: November 3, 2020
[Effect of puerarin on L-type calcium channel in isolated rat ventricular myocytes]
Xiao-gang Guo1, Jun-zhu Chen, Xiong Zhang
1Department of Cardiology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China. gxg@mail.hz.zj.cn
Objective:
To observe the effect of Puerarin on L-type calcium channel in isolated rat ventricular myocytes.
Method:
The cardiac ventricular myocytes were isolated enzymatically by Langendorff perfusion techniques at constant flow rate. Whole-cell recording of patch-clamp techniques was used to observe the current of L-type calcium channel.
Result:
Puerarin 2.4 mmol x L(-1) could inhibit the current of L-type calcium channel of rat ventricular myocytes and this inhibition was time-dependent. Purerarin elevated the current-voltage (I-V) curve of calcium current.
Conclusion:
Puerarin can inhibit L-type calcium current of rat ventricular myocytes. Which implies that puerarin takes part in anti-myocardial ischemia and anti-arrhythmics partly due to the inhibition of L-type calcium channel.

