Related Experiment Video
Updated: Aug 19, 2026
Murine Aortic Crush Injury: An Efficient In Vivo Model of Smooth Muscle Cell Proliferation and Endothelial Function
Published on: June 11, 2017
[Effect of puerarin and daidzein on proliferating vascular smooth muscle cells]
Jing Han1, Wei Wang, Lu-ya Wang
1Dongzhimen Hospital, University of Traditional Chinese Medicine, Beijing 100029, China.
Objective:
To observe the different effects of Puerarin and Daidzein on the expression of proliferating vascular smooth muscle cells, and to discuss the mechanism.
Method:
MT was used to detect the state of VSMC (vascular smooth muscle cell) activity. The expression levels of Survivin, Bcl-xl, Bax and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) messenger RNA (mRNA) were analyzed quantitatively by reverse transcriptase polymerase chain reaction (Rt-PCR).
Result:
Compared with Puerarin groups, VSMC activity in daidzein groups was lower, and the ratio of Bax/Gapdh/Bcl-xl/Gapdh was higher.
Conclusion:
The inhibition effect of daidzein on VSMC proliferation is stronger than that of puerarin.
