Related Experiment Video

Updated: Aug 19, 2026

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice
15:43

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice

Published on: March 17, 2014

Chronic pneumonia with Pseudomonas aeruginosa and impaired alveolar fluid clearance

Sophie Boyer1, Karine Faure, Florence Ader

  • 1Laboratoire de recherche en Pathologie Infectieuse, EA 2689. Faculté de Médecine de Lille, 59031 Lille Cedex, France. boyer.s@voila.fr

Respiratory Research
|February 15, 2005
PubMed
Abstract

Insights

Chronic Pseudomonas aeruginosa lung infection impairs distal alveolar fluid clearance (DAFC) in rats. This impaired DAFC response, even after pathogen eradication, may increase susceptibility to further lung injury.

Area of Science:

  • Pulmonary Medicine
  • Infectious Diseases
  • Respiratory Physiology

Background:

  • Limited understanding of chronic pneumonia's functional impact.
  • Focus on evaluating chronic Pseudomonas lung infection consequences on alveolar function.

Purpose of the Study:

  • To assess the impact of chronic Pseudomonas aeruginosa infection on alveolar function in a rat model.
  • To investigate the dynamics of alveolar-capillary barrier permeability and lung fluid clearance.

Main Methods:

  • Intratracheal instillation of Pseudomonas aeruginosa in agar beads in Sprague Dawley rats.
  • Measurement of alveolar-capillary barrier permeability, lung liquid clearance (LLC), and distal alveolar fluid clearance (DAFC) using radiolabeled albumin tracers.
  • Analysis conducted from day 2 to 21 post-infection, with a control group receiving sterile agar beads.

Main Results:

  • Alveolar-capillary permeability and LLC increased initially, returning to baseline by day 5.
  • Distal alveolar fluid clearance (DAFC) increased initially but returned to baseline by day 5, independent of TNF-alpha or catecholamines.
  • DAFC remained unresponsive to terbutaline stimulation and did not recover after ceftazidime treatment, despite pathogen persistence.

Conclusions:

  • Initial alveolar response (increased DAFC) to permeability is adequate but not sustained beyond day 5.
  • Impaired DAFC response in chronic pneumonia may contribute to increased susceptibility to subsequent lung injury.
  • Chronic Pseudomonas infection leads to a persistent, unresponsive defect in distal alveolar fluid clearance.

More Related Videos

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients07:25

Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients

Published on: July 16, 2020

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection06:56

A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection

Published on: February 20, 2020

Related Concept Videos

Pneumonia I: Introduction01:29

Pneumonia I: Introduction

Pneumonia is an infection of the lower respiratory tract that leads to inflammation of the lung parenchyma, often resulting in the accumulation of inflammatory exudate in the alveoli and airways. Unlike the watery, low-protein fluid exudate in pulmonary edema, the exudate in this case is a thick fluid rich in immune cells, proteins, and debris produced during infection and inflammation.This impairs gas exchange and can lead to consolidation of lung tissue. The infection may be caused by a...
Pneumonia I: Introduction01:30

Pneumonia I: Introduction

Pneumonia is an acute respiratory infection that targets the lungs, specifically the alveoli. These tiny air sacs, essential for oxygen exchange, become engorged with pus and fluid, severely hindering breathing, decreasing oxygen absorption, and causing significant pain and discomfort during respiration.
Risk Factors
Various factors influence the likelihood of developing pneumonia. Age plays a crucial role, with infants, children under two, and individuals over 65 at increased risk due to their...
Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
Atypical Pneumonia01:14

Atypical Pneumonia

Atypical pneumonia, often caused by Mycoplasma pneumoniae, is a form of pulmonary infection that differs from the classical presentation of bacterial pneumonia in both its cause and clinical symptoms. Mycoplasma pneumoniae is a pleomorphic bacterium notable for its lack of a rigid cell wall. This structural characteristic imparts resistance to beta-lactam antibiotics and significantly influences the bacterium’s behavior within the human host.Other pathogens responsible for the disease include...
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features01:24

Chronic Obstructive Pulmonary Disease III: Chronic Bronchitis Features

Chronic bronchitis is a key phenotype of chronic obstructive pulmonary disease (COPD), characterized by airway-centered inflammation and mucus overproduction. It develops from long-term exposure to harmful particles or gases, most commonly cigarette smoke, which triggers a persistent inflammatory response.Cellular and Structural ChangesInflammation initially affects the large bronchi and later the smaller airways, with infiltration by immune cells, including neutrophils, macrophages, and...