Functional genomics using high-throughput RNA interference
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Updated: Aug 19, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
Dominique Vanhecke1, Michal Janitz
1Max Planck Institute for Molecular Genetics, Department of Vertebrate Genomics, Fabeckstrasse 60-62, 14195 Berlin, Germany.
Abstract:
RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
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