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Updated: Aug 19, 2026

HPLC Measurement of the DNA Oxidation Biomarker, 8-oxo-7,8-dihydro-2’-deoxyguanosine, in Cultured Cells and Animal Tissues
Published on: August 1, 2015
Low total antioxidant status is implicated with high 8-hydroxy-2-deoxyguanosine serum concentrations in
Kleopatra H Schulpis1, Stylianos Tsakiris, Joanne Traeger-Synodinos
1Institute of Child Health, Aghia Sophia Children's Hospital, 11527 Athens, Greece.
Background:
Phenylketonuria (PKU), an inborn error of metabolism, is treated with a low phenylalanine (Phe) lifelong diet, which can be characterized as vegetarian. 8-Hydroxy-2-deoxyguanosine (8-OHdG) is highly implicated in degenerative diseases.
Objective:
To evaluate the effect of plasma total antioxidant status (TAS) and Phe on the serum marker of DNA damage, 8-OHdG, in PKU.
Methods:
Twenty-four PKU patients on a strict diet (group A), 25 PKU patients on a "loose diet" (group B), and 24 healthy children (controls) participated in this study. Plasma TAS was evaluated spectrophotometrically. 8-OHdG and Phe were measured in blood with immunoassays.
Results:
TAS levels were significantly higher (P < 0.001) in group A (1458 +/- 140 micromol/L) and controls (1452 +/- 235 micromol/L) than those in group B (907 +/- 150 micromol/L). In contrast, 8-OHdG serum levels were 2-fold higher in group B (0.22 +/- 0.03 ng/mL) as compared with those in group A (0.11 +/- 0.02 ng/mL) and 3-fold higher than those in controls (0.08 +/- 0.02 ng/mL) (P < 0.001). As expected, Phe levels were also significantly higher in group B than those in the other study groups. Positive correlation coefficients were found between Phe and 8-OHdG levels, whereas negative correlations were evaluated between TAS and 8-OHdG in all groups.
Conclusions:
The high Phe and the low TAS plasma levels in PKU patients on a "loose diet" may induce DNA oxidation, as evidenced by the measured high 8-OHdG level in their sera. 8-OHdG evaluation may be a useful marker of increased risk for a neurodegenerative process.
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