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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus
11:28

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

Human cytomegalovirus tegument protein ppUL35 is important for viral replication and particle formation

Karina Schierling1, Christopher Buser, Thomas Mertens

  • 1Abteilung Virologie, Universitätsklinikum Ulm, Albert Einstein Allee 11, D-89081 Ulm, Germany. michael.winkler@medizin.uni-ulm.de

Journal of Virology
|February 15, 2005
PubMed

Insights

Human cytomegalovirus (HCMV) tegument protein ppUL35 is essential for efficient viral replication. Deleting UL35 impairs immediate-early gene expression and virus assembly, highlighting ppUL35's critical role.

Area of Science:

  • Virology
  • Molecular Biology

Background:

  • Human cytomegalovirus (HCMV) tegument proteins ppUL35 and ppUL82 (pp71) interact to activate major immediate-early transcription.
  • The role of ppUL82 in HCMV growth is understood to be multiplicity of infection (MOI)-dependent, but ppUL35's significance remains unclear.

Purpose of the Study:

  • To investigate the biological significance and function of the HCMV tegument protein ppUL35.

Main Methods:

  • Generation of an HCMV mutant virus with a deletion in the UL35 gene.
  • Analysis of viral replication, immediate-early gene expression, and virus assembly at different MOIs.
  • Characterization of viral particle ultrastructure.

Main Results:

  • HCMV UL35 deletion resulted in a 1,000-fold reduction in progeny virus yield at MOI 0.1, and was essential at MOI 0.01.
  • Mutant virus exhibited reduced immediate-early gene expression and delayed lytic gene expression when standardized by viral DNA.
  • Defects in virus assembly were observed, with fewer enveloped particles and absent dense bodies in infected cells.

Conclusions:

  • ppUL35 plays a crucial role in HCMV immediate-early gene expression.
  • ppUL35 is important for efficient HCMV virus assembly.
  • The tegument protein ppUL35 is essential for optimal HCMV replication and infectivity.

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