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Entry of pseudorabies virus: an immunogold-labeling study
Harald Granzow1, Barbara G Klupp, Thomas C Mettenleiter
1Friedrich-Loeffler-Institut, Boddenblick 5A, 17493 Greifswald-Insel Riems, Germany. mettenleiter@rie.bfav.de
Journal of Virology
|February 15, 2005
Summary
Herpesviruses deliver their capsids into cells, losing most tegument proteins. Three specific proteins (UL36, UL37, US3) remain, aiding capsid transport to the nucleus.
Area of Science:
- Virology
- Cell Biology
- Molecular Biology
Background:
- Herpesviruses infect cells via membrane fusion, releasing nucleocapsids.
- Nucleocapsids traverse the cytoplasm along microtubules to reach the nucleus.
Purpose of the Study:
- To investigate which herpesvirus tegument proteins remain associated with the capsid after cell entry.
- To identify potential viral proteins involved in nucleocapsid transport.
Main Methods:
- Immunoelectron microscopy was used to examine the herpesvirus penetration process.
- Analysis focused on the association of major tegument proteins with intracytoplasmic capsids.
Main Results:
- The UL36, UL37, and US3 tegument proteins were found associated with capsids post-penetration.
- Tegument proteins UL11, UL47, UL48, and UL49 were lost during capsid entry.
Conclusions:
- UL36, UL37, and US3 are key capsid-associated tegument proteins.
- These proteins are likely involved in interacting with cellular motor proteins for nuclear transport.