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Mutational study of the 1p located genes p18ink4c, Patched-2, RIZ1 and KIF1B in oligodendrogliomas

M Eva Alonso1, M Josefa Bello, Dolores Arjona

  • 1Departamento de Cirugía Experimental, Laboratorio de Oncogenética Molecular, Unidad de Investigación, Hospital Universitario La Paz, Po. de la Castellana 261, 28046 Madrid, Spain. mevaalonso@gmx.net

Oncology Reports
|February 16, 2005
PubMed

Insights

Researchers investigated four genes on chromosome 1p, including Patched-2, RIZ1, KIF1B, and p18(ink4c), in oligodendroglioma tumors. No mutations were found, suggesting these genes are not the primary cause of 1p loss in oligodendroglioma development.

Area of Science:

  • Neuro-oncology
  • Cancer Genetics
  • Molecular Biology

Background:

  • Loss of heterozygosity on chromosome 1p is a hallmark of oligodendrogliomas.
  • Previous studies have failed to identify the specific tumor suppressor gene responsible for 1p loss in these brain tumors.

Purpose of the Study:

  • To investigate the role of novel oncosuppressor genes Patched-2, RIZ1, KIF1B, and p18(ink4c) located at 1p in oligodendroglioma development.
  • To determine if mutations in these genes correlate with 1p allelic status in oligodendrogliomas.

Main Methods:

  • Analysis of allelic status at 1p in 40 oligodendroglioma samples.
  • Detection of sequence variations in Patched-2, RIZ1, KIF1B, and p18(ink4c) using Polymerase Chain Reaction/Single-Strand Conformation Polymorphism (PCR/SSCP) methods.

Main Results:

  • No pathogenic mutations or significant sequence changes were detected in any of the four analyzed genes (Patched-2, RIZ1, KIF1B, p18(ink4c)).
  • The allelic status at 1p was analyzed in conjunction with gene sequencing results.

Conclusions:

  • The studied genes (Patched-2, RIZ1, KIF1B, p18(ink4c)) do not appear to be the target genes affected by mutations driving oligodendroglioma development via 1p loss.
  • Further research is needed to identify the specific gene(s) responsible for the characteristic 1p allelic loss in oligodendrogliomas.

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