[Fast purification of human serum albumin with chromatographic pie in analytical scale]

The purification of human serum albumin (HSA) by using analytical chromatographic pie was developed. Six standard proteins were separated on an analytical chromatographic pie. The results from the resolution of the standard proteins under various flow rates indicate that the chromatographic pie in analytical scale packed with small particles can be utilized under fast flow conditions. The type of mobile phase, concentration of (NH4)2SO4 and pH value for HSA purification were optimized. Crude HSA solution was purified in 10 min in analytical scale with the purity of HSA more than 85% and recovery of 65%. The results show that analytical chromatographic pie is suitable for fast separation and purification of biopolymers.