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[Direct enantiomeric separation of pantoprazole sodium by high performance liquid chromatography]
Guosheng Ding1, Runzi Cong, Junde Wang
1Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116012, China.
Abstract:
By using the Kromasil CHI-TBB chiral stationary phase, the racemic mixture of pantoprazole sodium was resolved in the normal phase mode. The influences of the composition of the mobile phase, the content of acidic and basic additives and the flow rate on this enantiomer separation were studied. The chiral high performance liquid chromatographic method for the analysis of pantoprazole sodium was established with high efficiency and good repeatability, by using hexane-isopropanol-acetic acid mixture (95:5:0.1, v/v) as the mobile phase at a flow rate of 2.0 mL/min at 25 degrees C.
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