[Determination of acteoside in Mitonghua particles and Brandisia hancei by high performance liquid chromatography]

A high performance liquid chromatographic method was established for the determination of acteoside in Mitonghua particles and Brandisia hancei. The results showed that acteoside can be extracted more efficiently with butanol than with water. Therefore, both Mitonghua particles and Brandisia hancei were extracted with butanol. The extracts were analysed on an RP-18 column using a mixture of CH3OH-0.5% aqueous HAc (42:58, v/v) as the mobile phase with UV detection at 334 nm. A good linear response was observed within the range of 0.029-2.038 microg of analytes (r = 0.9998). The recoveries were 90.2%-93.8% and the relative standard deviation was 0.34% (n = 5). This rapid, simple, accurate, and reproducible method is proposed for the quality control of Mitonghua particles.