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[Determination of iohexol by high performance liquid chromatography]
Qiuyu Zhu1, Yong Gu, Feng Ding
1Department of Nephrology, Affiliated Huashan Hospital, Fudan University, Shanghai 200040, China.
Se Pu = Chinese Journal of Chromatography
|February 17, 2005
Summary
A new high-performance liquid chromatography method accurately quantifies iohexol in plasma. This fast and sensitive technique offers reliable results for iohexol determination.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
Context:
- Accurate quantification of contrast media like iohexol is crucial for pharmacokinetic studies and clinical monitoring.
- Existing methods may be time-consuming or lack the required sensitivity and reproducibility.
Purpose:
- To develop and validate a rapid, sensitive, and reproducible high-performance liquid chromatography (HPLC) method for determining iohexol concentrations in human plasma.
Summary:
- A novel HPLC method utilizing a narrow-bore column and a simple isocratic elution system was established for iohexol analysis.
- The method features a single-step plasma deproteinization with perchloric acid.
- It demonstrates excellent linearity (0.5–500 mg/L, R² > 0.9998), a low limit of detection (154 pg), and short run times (<5 min).
Impact:
- This validated method provides a highly efficient tool for precise iohexol measurement in biological samples.
- It supports improved pharmacokinetic profiling and therapeutic drug monitoring of iohexol.
- The method's sensitivity and speed facilitate routine clinical laboratory applications.