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Published on: January 2, 2013
36 kDa glycoprotein isolated from Rhus verniciflua Stokes fruit has a protective activity to glucose/glucose
Jeong-Hyeon Ko1, Sei-Jung Lee, Kye-Taek Lim
1Molecular Biochemistry Laboratory and Biodefensive Substances Group, Institute of Biotechnology, Chonnam National University, Kwangju, #521, 300 Yongbong-Dong 500-757, South Korea.
Abstract:
The present study was carried out to investigate the antioxidative and antiapoptotic activities of 36 kDa RVS glycoprotein isolated from Rhus verniciflua Stokes fruits (RVS) in NIH/3T3 cells in vitro. The results showed that the RVS glycoprotein has scavenging activities of free radicals and hydroxyl radicals, and protects from glucose/glucose oxidase (G/GO)-induced cellular damage in NIH/3T3 cells dose-dependently. For example, cell viability was 43.5% in the G/GO treatment alone, whereas it was 93.8% in the co-treatment with RVS glycoprotein (200 microg/ml). We also demonstrated that RVS glycoprotein inhibits activities of the nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) induced by G/GO, and prevents from G/GO-induced apoptosis in the NIH/3T3 cells. In this regard, the results in this study indicated that RVS glycoprotein has a strong antioxidative activity and an antiapoptotic effect through the modulation activities of NF-kappaB and AP-1in NIH/3T3 cells.
