Related Experiment Video
Updated: Aug 1, 2026
Characterization of Inflammatory Responses During Intranasal Colonization with Streptococcus pneumoniae
Published on: January 17, 2014
Dynamics of anti-M antibody response in a mouse model following intranasal infection with group A Streptococcus M-18
Paul Gladstone1, George Varghese1, K N Brahmadathan1
1Department of Clinical Microbiology, Christian Medical College & Hospital, Vellore - 632004, Tamil Nadu, India 2Department of Microbiology, Pushpagiri Medical College, Tiruvalla - 689 101, Kerala, India.
Abstract:
Dynamics of anti-M antibody response following intranasal infection with group A Streptococcus (GAS) M-18 were investigated in a Swiss albino mouse model. Mice arranged in three groups were inoculated intranasally with 2.0x10(7) c.f.u. ml-1 of GAS M-18 on 1, 2 alternate and 3 alternate days. Plasma collected from the retro-orbital plexus was tested for antibodies by an in-house indirect ELISA. The antibody titres of the plasma samples varied from 1 : 8 to 1 : 1024 in the 1 day dose, from 1 : 4 to 1 : 256 in the 2 day dose and from 1 : 4 to 1 : 128 in the 3 day dose. Peak titres were seen on day 42 or 56 and in all cases the titres had declined by day 84. Swiss albino mouse can thus serve as a useful animal model to study different aspects of type-specific anti-M immune responses against GAS disease when designing candidate streptococcal vaccines.
Insights
This study shows Swiss albino mice can model group A Streptococcus (GAS) intranasal infections. The anti-M antibody response in mice provides insights for developing new GAS vaccines.
Area of Science:
- Immunology
- Microbiology
- Vaccinology
Background:
- Group A Streptococcus (GAS) causes various infections.
- Understanding type-specific anti-M immune responses is crucial for vaccine development.
- Intranasal infection models offer insights into mucosal immunity.
Purpose of the Study:
- To investigate the dynamics of the anti-M antibody response after intranasal GAS M-18 infection in a mouse model.
- To evaluate the Swiss albino mouse as a model for studying anti-GAS immunity.
Main Methods:
- Swiss albino mice were intranasally inoculated with GAS M-18.
- Different inoculation schedules (1, 2, or 3 alternate days) were used.
- Plasma anti-M antibody titers were measured using indirect ELISA.
Main Results:
- Antibody titers varied based on the inoculation dose and schedule.
- Peak antibody titers were observed between day 42 and 56 post-infection.
- Antibody titers declined by day 84 in all experimental groups.
Conclusions:
- The Swiss albino mouse model is suitable for studying type-specific anti-M immune responses to GAS.
- This model can aid in the design and evaluation of candidate streptococcal vaccines.
- Further research can explore different GAS serotypes and infection routes.
