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[Analysis of ant oil extracted with supercritical CO2 fluid from Polyrhachis vicina by GC-MS]
Li-Rong Shen1, Yu-Cui Ren, Feng-Qin Feng
1Department of Food Science & Nutrition, Zhejiang University, Hangzhou 310029, China. shenlirong@zju.edu.cn
Objective:
To study the optimum extraction parameters and components on ant oil from Polyrhachis vicina.
Method:
The optimum condifious for supercritical CO2 fluid extraction (SFE-CO2), were investigated with orthogonal design, GC-MS was applied for analyzing. The components and their contents in the ant oil were analyzed by GC-MS, and the contents of lead, zinc and manganese in the oil were determined by ICP-AES.
Result:
The optimum extraction parameters were achieved, temperature of 50 degrees C, pressure of 30 MPa and time of 2 hours. The extracting yield of the ant volatile oil was 11.4% - 14.3%. 51 Constituents were identified including 9-octadecenoic acid, ethyl oleate, cholesterol, n- Hexadecanoic acid, etc, and the content of various constituents was determined by orea normalization. The oil contained unsaturated fatty acid of 64.6%, lead of 0.80 microg x g(-1), zinc of 0.54 microg x g(-1) and manganese of 0.15 microg x g(-1).
Conclusion:
The method showes advantages including faster and efficient of extraction, good quality and no solvent residues in the oil.
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