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Published on: August 17, 2018
[Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro]
Xian-Feng Gong1, Min-Wei Wang, Shin-ichi Tashiro
1China-Japan Research Institute of Medical and Pharmaceutical University, Shenyang 110016, China.
Objective:
To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.
Method:
MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.
Result:
Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.
Conclusion:
The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
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