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Updated: Aug 19, 2026
Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications
Published on: November 17, 2015
[Freeze-drying of silymarin-loaded solid lipid nanoparticles (SM-SLN)]
Jun He1, Jian-fang Feng, Le-le Zhang
1West China School of Pharmacy, Sichuan University, Chengdu 610041, China.
Objective:
To investigate lyophilization of SM-SLN.
Method:
The parameters of lyophilization process was optimized. In addition, the protective effect of various types and concentrations of cryoprotectants were tested by shape, colour and disparity.
Result:
The mixture of 2% lactose and 2% glucose could better prevent nanoparticles from aggregating, the optimal lyophilization process was followed: precooled at -45 degrees C for 10 hr; primary drying at -25 degrees C for 5 hr; secondary drying at 10 degrees C for 3 hr; finally drying at 30 degrees C for 6 hr.
Conclusion:
Changes in particle size distribution during lyophilization could be minimized by optimizing the parameters of the lyophilization process and adding supporting agent.
