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Updated: Aug 19, 2026

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
Published on: April 17, 2020
[Recombinant analysis of classical swine fever virus]
Sheng Cao1, Chu-Yu Zhang, Guang-Hui Yi
1Animal Virus Division, Institute of Virology, Wuhan University, Wuhan 430072, China. sheng_cao@hotmail.com
Abstract:
To study the possible recombinant relationship among differently derived classical swine fever virus, the coding regions of 21 isolates were analyzed to detect recombination and breakpoints through gene trees comparison and quartet analyses. The results show nucleotide area corresponding to E0, E1 and E2 as a possible recombinant tract between ALD ( D49532) and GPE-(D49533) while NS5A-NS5B of the isolate 39 (AF407339) appears to be derived from a virulent Shimen strain (AF092448) sequence. This suggests that intertypic exchanges of genetic materials during mixed infections under natural or laboratorial conditions can lead classical swine fever virus to adapt to the changes of host environment.
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