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Related Experiment Videos

Cataracts in experimentally diabetic mouse: morphological and apoptotic changes.

K R Hegde1, S D Varma

  • 1Departments of Ophthalmology and Biochemistry, University of Maryland School of Medicine, Baltimore, MD 21201, USA.

Diabetes, Obesity & Metabolism
|February 18, 2005
PubMed
Summary

Diabetic cataract research in mice reveals cellular changes in lens epithelial and fiber cells. This mouse model, with low aldose reductase (AR) activity, is suitable for studying cataractogenesis and testing antioxidants.

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Area of Science:

  • Ophthalmology
  • Diabetology
  • Cell Biology

Background:

  • Diabetic cataracts are a significant complication of diabetes mellitus.
  • Previous research focused on biochemical aspects of diabetic cataractogenesis.
  • Aldose reductase (AR) plays a role in diabetic complications, with varying activity across species.

Purpose of the Study:

  • To investigate the morphological changes in the lenses of diabetic mice, a low aldose reductase (AR) animal model.
  • To extend previous biochemical studies to a morphological level.
  • To assess the suitability of this mouse model for cataractogenesis research.

Main Methods:

  • Diabetes was induced using streptozotocin in mice.
  • Isolated lenses underwent histological and electron microscopic examination.

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  • Morphological alterations in epithelial and fiber cells were analyzed.

    • Main Results:

    • Observed nuclear shrinkage, elongation, and lobulization in epithelial cells, with chromatin condensation.
    • Detected defects in fiber maturation, evidenced by structural aberrations.
    • Found abnormally nucleated cells in the posterior subcapsular region, similar to human diabetic cataracts.

    Conclusions:

    • The mouse model with low AR activity is suitable for studying diabetic cataractogenesis morphologically.
    • Findings support the use of this model over high-AR rat models for understanding cataract etiology.
    • This model is valuable for evaluating antioxidants against diabetic cataracts due to biochemical similarities with humans.