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Updated: Aug 19, 2026

Detection of Tilapia Lake Virus Using Conventional RT-PCR and SYBR Green RT-qPCR
Published on: November 10, 2018
A second-generation genetic linkage map of tilapia (Oreochromis spp.)
Bo-Young Lee1, Woo-Jai Lee, J Todd Streelman
1Hubbard Center for Genome Studies, University of New Hampshire, Durham, 03824, USA.
Abstract:
We constructed a second-generation linkage map of tilapia from the F(2) progeny of an interspecific cross between Oreochromis niloticus and Oreochromis aureus. The map reported here contains 525 microsatellite and 21 gene-based markers. It spans 1311 cM in 24 linkage groups, for an average marker spacing of 2.4 cM. We detected associations of sex and red color with markers on linkage group 3. This map will enable mapping and selective breeding of quantitative traits important to the economic culture of tilapia as a food fish and will contribute to the study of closely related cichlids that have undergone explosive adaptive radiation in the lakes of East Africa.

