Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
M-Cdk Drives Transition Into Mitosis02:15

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Metabolic buffering restricts phenotype switching in melanoma.

The EMBO journal·2026
Same author

AhR-dependent ferroptosis as a therapeutic opportunity to counteract BRAFi-resistance in melanoma.

Cell death discovery·2026
Same author

A Short Report on Melanocyte/Melanoma Culture, Senescence, and Reproducibility.

Pigment cell & melanoma research·2026
Same author

Dissecting the Impact of α-MSH-MC1R-cAMP Signaling on UVA-Induced Stress in Fibroblasts - Implications for Regulation of Cutaneous Photoaging.

Aging and disease·2026
Same author

[Melanoma and estrogens: A revolution in understanding metastatic mechanisms].

Medecine sciences : M/S·2026
Same author

Insights into lysosome-related organelle biogenesis: melanosome as a model organelle.

Frontiers in cell and developmental biology·2026

Related Experiment Video

Updated: Jun 25, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Mitf cooperates with Rb1 and activates p21Cip1 expression to regulate cell cycle progression.

Suzanne Carreira1, Jane Goodall, Isil Aksan

  • 1Signalling and Development Laboratory, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 OTL, UK.

Nature
|February 18, 2005
PubMed
Summary

The microphthalmia-associated transcription factor (Mitf) acts as an anti-proliferative factor, inducing cell cycle arrest in melanocytes. Mitf activates p21(Cip1) expression, cooperating with Rb1 to control cell proliferation and differentiation.

More Related Videos

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Related Experiment Videos

Last Updated: Jun 25, 2026

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols
12:02

Studying Cell Cycle-regulated Gene Expression by Two Complementary Cell Synchronization Protocols

Published on: June 6, 2017

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Melanocyte and melanoma cell proliferation share regulatory controls, but a specific regulator remains unidentified.
  • The microphthalmia-associated transcription factor (Mitf) plays a vital role in melanoblast and melanocyte survival and differentiation.

Purpose of the Study:

  • To investigate the role of Mitf in cell cycle regulation within the melanocyte lineage.
  • To identify key regulators of cell cycle progression specific to melanocytes.

Main Methods:

  • Investigated Mitf's function as a transcription factor.
  • Analyzed Mitf-mediated activation of the p21(Cip1) (CDKN1A) gene.
  • Examined the cooperation between Mitf and the retinoblastoma protein (Rb1).

Main Results:

  • Mitf functions as an anti-proliferative transcription factor.
  • Mitf induces a G1 cell-cycle arrest dependent on p21(Cip1) activation.
  • Cooperation between Mitf and Rb1 enhances Mitf's transcriptional activity, leading to cell cycle exit and differentiation.

Conclusions:

  • Mitf-mediated activation of p21(Cip1) and subsequent hypophosphorylation of Rb1 are crucial for cell cycle exit and differentiation.
  • Mutations in melanoma-associated genes like INK4a or BRAF can impair Mitf-mediated cell cycle control by affecting Mitf-Rb1 cooperation or Mitf stability.