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[Determination of gallic acid in Melastoma dodecandrum by RP-HPLC]
Xun He1, Yong-jun Li, Li-na Liu
1School of Pharmacy, Guiyang Medical College, Guiyang 550004, China. hexun224@gmc.edu.cn
Objective:
To establish a RP-HPLC method for the determination of gallic acid in Chinese herb Melastoma dodecandrum, and to evaluate the quality of the herb.
Method:
The RP-HPLC analysis was achieved by using a Polaris C18 column and tetrahydrofuran-methanol-0.2% phosphoric acid (0.5:0.5:99) as the mobile phase, with a flow rate of 1.0 mL x min(-1), and detected by UV at 274 nm.
Result:
The content of gallic acid was from 0.020% to 0.081%, in ten groups of M. dodecandrum collected from different locations.
Conclusion:
The method is a simple, convenient and rapid. It can be used for quality evaluation of M. dodecandrum.
