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[Determination of linarin in Yuye Jiedu granule by RP-HPLC]
Dan Lu1, Gao-rong Lu, Jie-ming Zou
1Guilin Sanjin Pharmaceutical Co. Ltd., Guilin 541004, China.
Objective:
To determine the content of linarin in Yuye Jiedu granule.
Method:
A HPLC method was developed. The chromatographic conditions are as follows Luna C18 column and acetonitrile-0.05 mol x L(-1) phosphate buffer-phosphoric acid (30:70:0.06) as mobil phase, detection wavelenth at 327 nm.
Result:
The linear range of linarin was 0.025-0.50 microg. The average recovery was 98.7% and RSD 2.7%.
Conclusion:
The method is simple and accurate, with good repeatability, and can be used for determination of linarin in Yuye Jiedu granule.
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