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Migration of polymorphonuclear leucocytes is influenced by dendritic cells
M Lucila Scimone1, Viviana P Lutzky, Sandra I Zittermann
1Laboratorio de Inmunogenética, Hospital de Clínicas José de San Martín, Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Immunology
|February 22, 2005
Summary
Dendritic cells (DCs) initiate innate immune responses by recruiting polymorphonuclear leucocytes (PMNLs) through CXCL8 production. This process is modulated by CD31 signaling during DC migration, influencing PMNL chemotaxis.
Area of Science:
- Immunology
- Cell Biology
- Inflammation Research
Background:
- Dendritic cells (DCs) are potent antigen-presenting cells involved in inflammatory reactions.
- Polymorphonuclear leucocyte (PMNL) infiltration is a hallmark of inflammation, driven by chemotactic factors and endothelial adhesion molecules.
Purpose of the Study:
- To investigate the role of DCs in the early recruitment of PMNLs.
- To determine if monocyte-derived DCs (moDCs) influence PMNL recruitment and migration.
Main Methods:
- Monocyte-derived DCs (moDCs) were cultured and treated with tumor necrosis factor-alpha (TNF-alpha).
- PMNLs were incubated with moDC culture supernatants (CS).
- Chemokine blocking assays and adhesion molecule engagement experiments were performed.
Main Results:
- moDC culture supernatants induced PMNL surface expression of CD11b and CD18, and enhanced PMNL chemotaxis.
- CXCL8 (interleukin-8) was identified as a key chemokine mediating PMNL recruitment.
- Engagement of CD31 on immature moDCs reduced CXCL8 production, suggesting regulation during endothelial migration.
Conclusions:
- DCs contribute to the innate immune response by recruiting PMNLs, in addition to initiating specific immune responses.
- CXCL8 production by immature DCs is regulated by CD31 signaling during vascular endothelium transmigration.