Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Image-based screening: a technology in transition.

Peter Ramm1

  • 1Invicta Research Inc., 290 Riverview Boulevard, St Catharines, ON L2T 3N4, Canada. Peter.Ramm@invictaresearch.com

Current Opinion in Biotechnology
|February 22, 2005
PubMed
Summary

Subcellular image-based screening (IBS) uses automated imaging for drug discovery. While promising for early-stage lead quality, this technology requires further development for robust application.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Automated screening of neurite outgrowth.

Journal of biomolecular screening·2003
Same author

Image-based screening of signal transduction assays.

Science's STKE : signal transduction knowledge environment·2003
See all related articles

Area of Science:

  • Biotechnology
  • Drug Discovery
  • Cell Biology

Background:

  • Image-based screening (IBS) is established for whole-well assays, prioritizing throughput and miniaturization.
  • Current research focuses on automated imaging for subcellular resolution assays in drug discovery.
  • Subcellular IBS aims to enhance lead quality in early-stage drug development.

Purpose of the Study:

  • To review the current state and potential of subcellular image-based screening (IBS).
  • To highlight the ongoing technological advancements in subcellular IBS.

Main Methods:

  • Review of automated imaging technologies for cellular assays.
  • Analysis of current applications and limitations of subcellular IBS.
  • Discussion of ongoing developments in image acquisition, analysis, and informatics.

Main Results:

  • Whole-well IBS is mature, but subcellular IBS is still developing.
  • Some subcellular assays offer reliable data at good throughput.
  • Many subcellular IBS applications require further validation for robustness.

Conclusions:

  • Subcellular IBS holds significant potential for improving early-stage drug discovery.
  • Continued advancements in imaging and analysis technologies are crucial.
  • Further development is expected to expand the utility and reliability of subcellular IBS.

Related Experiment Videos