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Isolation of fibronectin type III like peptide from human placental extract used as wound healer
Piyali Datta Chakraborty1, Debasish Bhattacharyya
1Department of Drug Design, Development and Molecular Modeling, Indian Institute of Chemical Biology, 4 Raja S.C. Mallick Road, Jadavpur, Calcutta, West Bengal 700032, India. p_dattach@rediffmail.com
Abstract:
A peptide of around 7.4 kDa has been purified from the aqueous extract of human placenta used as wound healer. Derived partial amino acid sequence from mass spectrometric analysis showed its homology with human fibronectin type III. Under nondenaturing condition, it formed aggregate, the elution pattern of which from reverse-phase HPLC was identical with that of fibronectin type III. Immuno-blot of the peptide with reference fibronectin type III-C showed strong cross reactivity. Since fibronectin type III plays important roles in wound healing, similar peptide in the extract is likely to take part in curing process.
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