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Developmental decrease in rat small intestinal creatine uptake.

M J Peral1, M Gálvez, M L Soria

  • 1Departamento de Fisiología y Zoología, Facultad de Farmacia, Universidad de Sevilla, C/Garcia Gonzalez no. 2, 41012 Sevilla, Spain.

Mechanisms of Ageing and Development
|February 22, 2005
PubMed
Summary

Rat intestinal creatine transport activity changes with maturation. This energy buffer uptake is highest in newborns, mediated by a single system, and influenced by diet and genetics.

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Area of Science:

  • Physiology
  • Biochemistry
  • Molecular Biology

Background:

  • Phosphocreatine serves as a vital energy buffer and transducer in key tissues like the heart, brain, and skeletal muscle.
  • The Na+/Cl-/creatine transporter has been identified at the apical membrane of the small intestinal epithelium, suggesting a role in creatine uptake.

Purpose of the Study:

  • To investigate the developmental changes (ontogeny) and regional differences (segmental distribution) of creatine transport activity in the rat small intestine.
  • To elucidate the characteristics of the creatine transport system in different segments of the rat intestine across various life stages.

Main Methods:

  • Quantification of [14C]-creatine uptake in the jejunum and ileum of rats at multiple developmental stages, from fetal to adult.
  • Measurement of creatine content in maternal and infant diets (amniotic fluid, milk, rat chow) using High-Performance Liquid Chromatography (HPLC).

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Main Results:

  • NaCl-dependent creatine uptake was highest in newborn rats and consistently higher in the ileum than the jejunum across all ages studied.
  • Creatine uptake in the jejunum became undetectable after weaning, while ileal uptake showed a periweaning decline that could be partially mitigated by creatine supplementation.
  • Kinetic analysis indicated that both jejunum and ileum utilize the same creatine transport system, with maturation primarily affecting Vmax rather than apparent Km.

Conclusions:

  • The rat small intestine employs a single transport system for creatine uptake along its length.
  • Creatine transport activity undergoes significant, maturation-dependent changes, appearing genetically programmed and regulated by intestinal creatine levels.
  • Dietary creatine supplementation can influence intestinal creatine uptake, particularly in the ileum, highlighting the interplay between diet and intestinal physiology.