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Related Experiment Videos

A novel pH- and ionic-strength-sensitive carboxy methyl dextran hydrogel.

Rhongsheng Zhang1, Mingguo Tang, Adrian Bowyer

  • 1Department of Chemical Engineering, University of Bath, Claverton Down Bath, Banes BA2 7AY, UK.

Biomaterials
|February 22, 2005
PubMed
Summary

Researchers developed pH-sensitive hydrogel membranes for drug delivery using carbodiimide chemistry. These adaptable hydrogels show tunable porosity and reversible permeability, crucial for advanced biomedical applications.

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Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Drug Delivery Systems

Background:

  • Developing responsive materials is key for advanced drug delivery and tissue engineering.
  • Hydrogels offer versatile platforms due to their tunable properties.
  • pH-sensitive materials are particularly valuable for targeted release.

Purpose of the Study:

  • To create a fast and simple method for preparing pH-sensitive hydrogel membranes.
  • To investigate the properties and potential applications of these hydrogels in drug delivery and tissue engineering.

Main Methods:

  • Carboxymethyl dextran (CM-dextran) was cross-linked using carbodiimide chemistry (EDC/NHS).
  • Infrared spectroscopy confirmed ester bond formation.
  • Protein diffusion assays assessed porosity and permeability.

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  • Scanning electron microscopy visualized structural changes.

    • Main Results:

    • Hydrogel membranes exhibited pH-dependent swelling and porosity.
    • Protein (lysozyme) diffusion increased with pH (5.0-9.0) and ionic strength.
    • The observed changes in permeability and structure were reversible.
    • Ester bond formation was confirmed via infrared spectroscopy.

    Conclusions:

    • A facile method for producing pH-sensitive hydrogel membranes was established.
    • The hydrogels demonstrate tunable permeability responsive to pH and ionic strength.
    • These findings support the potential of these hydrogels for controlled drug delivery and tissue engineering applications.