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Published on: April 1, 2015
Oxidatively modified fibrinogen modulates blood rheological parameters
E V Roitman1, O A Azizova, Yu A Morozov
1Russian Scientific Center of Surgery, Russian Academy of Medical Sciences, Moscow, Russia.
Bulletin of Experimental Biology and Medicine
|February 22, 2005
Summary
UV-oxidized fibrinogen negatively impacts blood rheology, with higher oxidation levels causing more severe changes. This study reveals how altered fibrinogen affects blood flow properties.
Area of Science:
- Biophysics
- Hematology
- Biochemistry
Background:
- Fibrinogen is a key protein in blood coagulation and rheology.
- Oxidative stress can modify protein structure and function.
- Understanding oxidative fibrinogen's impact on blood flow is crucial for cardiovascular health.
Purpose of the Study:
- To investigate the effects of UV-oxidized fibrinogen on blood rheological parameters.
- To determine the dose-dependent relationship between fibrinogen oxidation degree and rheological changes.
Main Methods:
- Blood samples were treated with UV-oxidized fibrinogen at 10% and 20% oxidation degrees.
- Rheological parameters including erythrocyte deformability, aggregate formation, blood viscosity, and suspension stability were measured.
- Caisson and asymptotic viscosities were analyzed.
Main Results:
- Fibrinogen with 10% oxidation showed moderate, variable effects, suggesting antioxidant compensation.
- Fibrinogen with 20% oxidation significantly decreased erythrocyte deformability, altered aggregate dynamics (delayed linear, accelerated 3D formation), and increased overall aggregate strength.
- Blood viscosity increased at all shear rates, suspension stability decreased, and the difference between Caisson and asymptotic viscosities widened, without changes in plasma viscosity or Caisson viscosity.
Conclusions:
- Oxidized fibrinogen induces detrimental changes in blood rheological properties.
- The severity of these rheological alterations is dependent on the degree of fibrinogen oxidation.
- These findings highlight potential risks associated with oxidative stress in cardiovascular function.
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