Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Conformations of co-translational folding intermediates.

Michael S Evans1, Thomas F Clarke, Patricia L Clark

  • 1Department of Chemistry and Biochemistry, University of Notre Dame, 251 Nieuwland Science Hall, Notre Dame, IN 46556, USA.

Protein and Peptide Letters
|February 23, 2005
PubMed
Summary

This review explores how proteins fold inside living cells, focusing on early folding stages during ribosome synthesis. It discusses cellular challenges like aggregation and crowding that impact protein folding.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deep Receptor Scanning Reveals General Sequence Constraints on GPCR Biosynthesis.

bioRxiv : the preprint server for biology·2025
Same author

Translon: a single term for translated regions.

Nature methods·2025
Same author

Identification of an on-pathway intermediate illuminates the kinetic competition between protein folding and misfolding.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Protein folding success depends on the direction and speed of polypeptide chain appearance.

bioRxiv : the preprint server for biology·2025
Same author

Guidelines for minimal reporting requirements, design and interpretation of experiments involving the use of eukaryotic dual gene expression reporters (MINDR).

Nature structural & molecular biology·2025
Same author

Discovery of an on-pathway protein folding intermediate illuminates the kinetic competition between folding and misfolding.

bioRxiv : the preprint server for biology·2025

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • In vitro studies provide foundational knowledge of protein folding.
  • Understanding in vivo protein folding remains a significant challenge.
  • Nascent polypeptides synthesized by the ribosome are key subjects.

Purpose of the Study:

  • To review current knowledge on early in vivo protein folding intermediates.
  • To discuss conformations adopted by nascent polypeptides during ribosomal synthesis.
  • To explore challenges of protein folding within the cellular environment.

Main Methods:

  • Literature review of in vivo protein folding studies.
  • Analysis of nascent polypeptide conformations.
  • Discussion of cellular factors influencing folding.

Related Experiment Videos

Main Results:

  • Identified earliest in vivo folding intermediates as conformations of nascent polypeptides.
  • Highlighted the ribosome's role in initiating protein folding.
  • Detailed challenges including off-pathway aggregation and macromolecular crowding.

Conclusions:

  • In vivo protein folding is complex and distinct from in vitro conditions.
  • Nascent polypeptide conformations are critical early folding events.
  • Cellular environment presents significant hurdles to successful protein folding.