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Updated: Aug 19, 2026

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
Published on: April 6, 2016
Efficient immobilization of enzymes on microchannel surface through His-tag and application for microreactor
Masaya Miyazaki1, Jun Kaneno, Susumu Yamaori
1Micro-Space Chemistry Laboratory, National Institute of Advanced Industrial Science and Technology (AIST), Saga, Japan.
Abstract:
We developed a simple immobilisation method for His-tagged enzymes on a microchannel surface. It facilitates immobilisation of protein molecule on microchannel surface through Ni-complex, using crude or purified protein solutions. By this method, we could immobilize proteins on microcapillary constantly. This method might be useful for further development of microreactor with reversibly immobilized enzymes.
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