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Updated: Aug 19, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
Published on: June 10, 2022
Plant genotype affects total antioxidant capacity and phenolic contents in fruit
Jessica Scalzo1, Alessia Politi, Nicoletta Pellegrini
1Dipartimento di Scienze Ambientali e delle Produzioni Vegetali, Università Politecnica delle Marche, Marche, Italy.
Objective:
We wanted to determine the total antioxidant capacity (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid equivalent antioxidant assay; TEAC) of different species and cultivars (strawberry and apple) and to characterize the TEAC among fruits of the same varieties (peach and apricot) but grafted on different rootstocks.
Methods:
The study was carried out with wild strawberry, six varieties of cultivated strawberry, and five varieties of apple and with apricot and peach grafted on five different rootstocks that are known to induce different plant vigor and fruit qualitative features. The TEAC (considering lipophilic and hydrophilic contributions) was determined for all fruit varieties, and total polyphenol contents were assayed. One-way analysis of variance was used to test differences among fruits and to identify possible correlations of TEAC versus total phenolics.
Results:
The following hierarchy of antioxidant activities was found: wild strawberries >> cultivated strawberries >> kiwifruit = apples = apricots = peaches. Further, differences were found among strawberries with different genotypes and in apricots and peaches grafted from different rootstocks.
Conclusions:
These observations suggest the importance of genotype for determining antioxidant potential and phenolic contents. Variety manipulation may be a powerful tool for modifying antioxidant fruit patterns and contents.
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