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[Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte]
Wei Gu1, Wen-heng Zeng, Hai-ying Hu
1The 2nd Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, China. guwei@aiocrm.com
Objective:
To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte.
Method:
The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference.
Result:
The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05).
Conclusion:
In vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine.
