Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene transfer into specific vascular cells.

J T Powell1, A J van Zonneveld, J A van Mourik

  • 1Central Laboratory of The Netherlands Red Cross, Amsterdam.

European Journal of Vascular Surgery
|March 1, 1992
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Attenuation of Torque teno viral load over time in kidney transplantation recipients treated with calcineurin inhibitors is mitigated after conversion to belatacept.

Journal of medical virology·2024
Same author

Conversion from calcineurin inhibitors to belatacept-based immunosuppressive therapy skews terminal proliferation of non-classical monocytes and lowers lymphocyte counts.

Transplant immunology·2024
Same author

[RNAissance: is there a future for RNA therapeutics?]

Nederlands tijdschrift voor geneeskunde·2020
Same author

A novel method for engineering autologous non-thrombogenic in situ tissue-engineered blood vessels for arteriovenous grafting.

Biomaterials·2019
Same author

Value of risk scores in the decision to palliate patients with ruptured abdominal aortic aneurysm.

The British journal of surgery·2018
Same author

Perfused 3D angiogenic sprouting in a high-throughput in vitro platform.

Angiogenesis·2018

Gene transfer therapy shows promise for vascular surgery. Lipofectin efficiently delivers genes to endothelial and smooth muscle cells, enhancing gene expression for potential therapeutic applications.

Area of Science:

  • Biotechnology
  • Vascular Surgery
  • Gene Therapy

Background:

  • Gene transfer therapy is emerging in vascular surgery.
  • Applications include anti-clotting proteins in grafts and controlling cell proliferation post-intervention.

Purpose of the Study:

  • To investigate non-infectious gene targeting methods for specific vascular cells.
  • To evaluate DEAE-dextran and lipofectin complexes for reporter gene transfection.

Main Methods:

  • Cultured human umbilical vein/artery endothelial cells, smooth muscle cells, and fibroblasts were used.
  • Firefly luciferase reporter gene with Rous sarcoma virus (RSV) and PAI-1 promoters were employed.
  • DEAE-dextran and lipofectin transfection methods were compared.

Related Experiment Videos

Main Results:

  • DEAE-dextran yielded low, transient gene expression (2-5 days).
  • Lipofectin significantly increased expression (4-5 fold) in endothelial and smooth muscle cells, stable for up to 14 days.
  • RSV promoter showed higher luciferase expression than PAI-1 in smooth muscle cells.

Conclusions:

  • Lipofectin is an effective non-viral vector for gene delivery to vascular cells.
  • This method holds potential for augmenting cultured vascular cells with foreign genes for therapeutic purposes.