Related Experiment Video
Updated: Aug 19, 2026
Isolation of Human Primary Valve Cells for In vitro Disease Modeling
Published on: April 16, 2021
[Mineralization of homograft aortic valves stored in a nutrient solution]
Grzegorz Goncerz1, Piotr Podolec, Eugeniusz Rokita
1Katedra Anatomii Collegium Medicum, Uniwersytet Jagielloński, Kraków.
Background And Aim Of The Study:
Mineralization of the homograft aortic valve cusps is a complex process leading to their degeneration and decreasing durability. Factors that play a decisive role in regulation of mineralization, growth of hydroxyapatite crystals are still open questions. We studied development of mineralization in the homograft aortic valves prepared for transplantation stored in the Parker's solution with antibiotics.
Method:
The aortic valves were explanted from 12 donors <18 hours after death. All the cusps were divided into halves. One half of each cusp was stored in the Parker's solution with antibiotics for 28 days at 4 degrees C. Concentrations of several elements (P, S, Cl, K, Ca, Mn, Fe, Ni, Cu, Zn i Sr) in incubated and control samples were determined using energy dispersive X-ray fluorescence (ED-XRF).
Results:
The study showed that the concentration of Ca and Ca/P increased during incubation that may indicate development of mineralization.
Conclusions:
The homograft aortic valve cusps underwent mineralization during in vitro incubation. This may aggravate mineralization in the aortic valve recipients and decrease valve durability which indicate a need for modification of storing condition.
