Terminal phosphate-labeled nucleotides with improved substrate properties for homogeneous nucleic acid assays

Anup Sood1, Shiv Kumar, Satyam Nampalli

  • 1GE Healthcare, 800 Centennial Avenue, Piscataway, New Jersey 08855, USA.

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The titration curve of a weak base like ammonia with a strong acid like hydrochloric acid is the mirror image of the titration curve of a weak acid with a strong base.
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Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
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