Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method allowing DIC imaging in conjunction with confocal microscopy.

S H Cody1, S D Xiang, M J Layton

  • 1Ludwig Institute for Cancer Research, PO Box 2008, Royal Melbourne Hospital, Victoria 3050, Australia. stephen.cody@ludwig.edu.au

Journal of Microscopy
|February 24, 2005
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scanning laser-induced endothelial injury: a standardized and reproducible thrombosis model for intravital microscopy.

Scientific reports·2022
Same author

The omics revolution: beyond genomics. A meeting report.

Clinical proteomics·2020
Same author

Mass Spectrometry-Based Analysis for the Discovery and Validation of Potential Colorectal Cancer Stool Biomarkers.

Methods in enzymology·2017
Same author

Experimental approaches to studying the nature and impact of splicing variation in zebrafish.

Methods in cell biology·2016
Same author

From proteomics to personalized medicine: the road ahead.

Expert review of proteomics·2016
Same author

HBV-induced ROS accumulation promotes hepatocarcinogenesis through Snail-mediated epigenetic silencing of SOCS3.

Cell death and differentiation·2016
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

A new, simple method enables simultaneous collection of confocal and contrast-enhanced images, such as differential interference contrast (DIC) or phase images, using a standard confocal laser scanning microscope (CLSM). This technique simplifies image acquisition without compromising confocal image quality.

Area of Science:

  • Microscopy
  • Optical Imaging
  • Biotechnology

Background:

  • Confocal laser scanning microscopy (CLSM) combined with transmitted light detection (TLD) for Nomarski differential interference contrast (DIC) or phase imaging is often complex and inefficient.
  • Existing methods require specialized hardware or optical adjustments, limiting accessibility and practicality.

Purpose of the Study:

  • To introduce a simplified method for acquiring simultaneous confocal and contrast-enhanced images (DIC, phase, Zernike phase, dark-field, Hoffman modulation contrast).
  • To enable contrast-enhanced image calculation from both live and archival data without compromising CLSM image quality.

Main Methods:

  • Utilized the commercial product QPm in conjunction with brightfield images from a CLSM's TLD.
  • Employed the quantitative phase-amplitude microscopy (QPm) technique for contrast-enhanced image calculation via software.

Related Experiment Videos

  • Required only a CLSM with a motorized z-drive and TLD, with no hardware modifications.

    • Main Results:

    • Successfully generated DIC, phase, Zernike phase, dark-field, and Hoffman modulation contrast images.
    • Demonstrated that contrast-enhanced imaging does not degrade the intensity or quality of simultaneously acquired confocal images.
    • Enabled DIC imaging of cells in plastic and without strain-free objectives, overcoming limitations of conventional DIC.

    Conclusions:

    • The QPm-based method offers a simple, efficient, and versatile approach to combine CLSM with various contrast-enhanced imaging techniques.
    • This technique allows researchers to focus on experimental procedures rather than complex optical setups.
    • It expands the applicability of DIC and phase imaging in microscopy, particularly for challenging samples.