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Coexpression and regulation of the FGF-2 and FGF antisense genes in leukemic cells

Mark Baguma-Nibasheka1, Audrey W Li, Mohammed S Osman

  • 1Department of Physiology and Biophysics, Faculty of Medicine, Dalhousie University, 5850 College Street, Halifax, Nova Scotia, Canada B3H 1X5.

Leukemia Research
|February 24, 2005
PubMed

Insights

Fibroblast growth factor-2 (FGF-2) and its antisense RNA (FGF-AS) are coordinately expressed in hematopoietic cells. This suggests both proteins play a role in lymphoid and myeloid tumor cell survival and proliferation.

Area of Science:

  • Molecular Biology
  • Hematology
  • Cancer Biology

Background:

  • Fibroblast growth factor-2 (FGF-2) is a key regulator of cell growth and survival.
  • Elevated FGF-2 expression is observed in various hematopoietic malignancies.
  • Natural antisense RNAs, like FGF-AS, are involved in posttranscriptional gene regulation.

Purpose of the Study:

  • To investigate the coordinated expression and translation of FGF-2 and FGF-AS in hematopoietic cells.
  • To elucidate the functional roles of FGF-2 and its antisense RNA in lymphoid and myeloid tumor cells.

Main Methods:

  • Analysis of FGF sense and antisense RNA expression in hematopoietic cells and tissues.
  • Cytokine stimulation of K562 cells to study mRNA and protein expression dynamics.
  • Assessment of FGF-2 and GFG/NUDT6 protein expression and subcellular localization.

Main Results:

  • Demonstrated coordinate expression and translation of FGF-2 and FGF-AS RNAs in hematopoietic cells.
  • Observed rapid, transient increase in FGF-AS mRNA followed by sustained FGF-2 mRNA increase upon cytokine stimulation.
  • Documented increased expression and nuclear translocation of FGF-2 and the FGF-AS encoded protein, GFG/NUDT6.

Conclusions:

  • FGF-2 and GFG/NUDT6 proteins are likely involved in the survival and proliferation of lymphoid and myeloid tumor cells.
  • The coordinated expression of FGF-2 and FGF-AS suggests a regulatory mechanism influencing hematopoietic malignancy progression.

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