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Related Experiment Videos

Are stem cell characteristics altered by disease state?

Anthony Kicic1, Christine M Hall, Wei-Yong Shen

  • 1Stem Cell Unit, Department of Molecular Ophthalmology, Lions Eye Institute and the University of Western Australia, Nedlands, 6009, Western Australia, Australia. anthonyk@ichr.uwa.edu.au

Stem Cells and Development
|February 24, 2005
PubMed
Summary

Mesenchymal stem cells (MSCs) from retinal degenerative mice show a significantly larger undifferentiated cell pool. These disease-derived MSCs can still differentiate and be gene-modified, offering therapeutic potential.

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Area of Science:

  • Stem Cell Biology
  • Regenerative Medicine
  • Genetics

Background:

  • Autologous stem cell transplantation combined with gene therapy holds promise for inherited diseases.
  • Characterizing multipotent cells from disease states is crucial but limited.
  • Adult bone marrow stromal cells (MSCs) are a potential source for such therapies.

Purpose of the Study:

  • To characterize and compare adult bone marrow stromal cells (MSCs) from retinal degenerative mouse models with those from normal counterparts.
  • To investigate the differentiation potential and gene transduction efficiency of disease-derived MSCs.

Main Methods:

  • Isolation and culture of bone marrow stromal cells (MSCs) from three retinal degenerative mouse models and normal controls.
  • Flow cytometry analysis (CD90, STRO-1) to assess differentiation status at 30 days post-isolation.

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  • Assessment of MSC differentiation into desired lineages and gene transduction efficiency using viral vectors.

    • Main Results:

    • MSCs from disease models exhibited a significantly higher percentage (89-99%) of undifferentiated CD90(+)/STRO-1(+) cells compared to normal counterparts (19-43%) at 30 days.
    • Fetal bovine serum supported MSC proliferation without inducing differentiation; alternatives like EGF, PDGF, and LIF were identified.
    • CD90(+)/STRO-1(+) cells from both disease and normal states demonstrated comparable differentiation rates and gene transduction efficiencies.

    Conclusions:

    • A substantial pool of undifferentiated stem cells exists in bone marrow stromal cells derived from a disease state.
    • These disease-derived stem cells retain the potential to differentiate into desired cell types when properly stimulated.
    • The findings support the potential application of these cells in autologous stem cell transplantation and gene therapy for inherited diseases.