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Updated: Aug 19, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Clinical and biological considerations regarding glioma based on the TUNEL method and immunohistochemical staining
1Department of Neurosurgery, Yamagata University School of Medicine Yamagata, 2-2-2 Iida Nishi, Yamagata 990-23, Japan.
Abstract:
We examined 36 gliomas both by the terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick-end-labeling (TUNEL) technique and by immunostaining of antibodies against apoptosis-related antigens. The apoptotic index (AI) obtained by the TUNEL method was 10.77 +/- 3.78% in astrocytomas, 6.19 +/- 1.81% in anaplastic astrocytomas, and 5.21 +/- 1.14% in glioblastomas. The AI of anti-Fas antibody and Le(Y) antibody in gliomas also showed a similar tendency as the results with the TUNEL method. As a result, statistically significant differences were observed in the distribution of survival between the two groups according to the AI obtained by the TUNEL method. Therefore, an analysis of the AI is considered to be useful for prognosis.
