Related Experiment Video
Updated: Aug 19, 2026

Isolation of Sertoli Cells and Peritubular Cells from Rat Testes
Published on: February 8, 2016
[Effects of p,p'-DDE on intracellular MAPK pathway of rat Sertoli cells in vitro]
Chong Wang1, Jiaojun Liang, Aiguo Wang
1Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Objective:
To study effects of p,p'-DDE on intracellular p-ERK of rat Sertoli cells in vitro.
Methods:
We cultured Sertoli cells separated from testicular tissue of rats and examined the changes of intracellular p-ERK, using immunohistochemical SABC method.
Results:
It was showed that intracellular p-ERK increased with the dose of p,p'-DDE and had a dose-dependent trend.
Conclusion:
The results suggested that p,p'-DDE could result in the phosphorylation of ERK and induce the activation of the downstream road and the transcription and expression of genes.
