Related Experiment Video
Updated: Aug 19, 2026

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Identification of strain specific markers in Bacillus anthracis by random amplification of polymorphic DNA
Haim Levy1, Morly Fisher, Naomi Ariel
1Department of Infectious Diseases, Israel Institute for Biological Research, PO Box 19, Ness-Ziona, Israel. levy@iibr.gov.il
Abstract:
Classification and differentiation of Bacillus anthracis isolates by genetic markers play an important role in anthrax research. We used a PCR based method--Random Amplification of Polymorphic DNA (RAPD)--to identify genetic markers in B. anthracis strains. Twenty-five differential genetic markers were identified which divided the strains into five different groups. Three selected RAPD-markers were cloned and sequenced. The five RAPD-derived genotypes could be defined by integration of these three markers. This system offers a simple non-expensive method to classify B. anthracis strains in laboratories involved in the research of this bacterium.
Related Concept Videos
Modern Molecular Taxonomy
Methods of Classification and Identification
Applications of Molecular Taxonomy
Rapid Identification of Pathogens
