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Updated: Aug 19, 2026
A Translational Surgical Porcine Model for Postoperative Intra-Abdominal Adhesion Formation
Published on: March 13, 2026
Prevention of intraperitoneal adhesions and abscesses by polysaccharides isolated from Phellinus spp in a rat
Jae-Sung Bae1, So-Jeo Ahn, Hyunee Yim
1Department of Surgery, College of Veterinary Medicine, Kangwon National University, Chunchon, Korea.
Objective:
To assess whether polysaccharides isolated from fungi, Phellinus spp, could reduce the adhesion and abscess formation in a rat peritonitis model.
Summary Background Data:
Although polysaccharides from Phellinus spp is a well-known material with antiinflammatory properties, little is known regarding its ability to prevent intraperitoneal adhesions. We have assessed the adhesion- and abscess-reducing effect of polysaccharides from Phellinus gilvus (PG) and Phellinus linteus (PL) in a rat peritonitis model.
Methods:
In 60 SD rats, experimental peritonitis was induced using the cecal ligation and puncture model (CLP). Animals were randomly assigned to 5 groups; ringer lactate solution (RL group), polysaccharides from PG and PL (PG and PL group), hyaluronic acid (HA group), and carboxymethylcellulose (CMC group). Intraperitoneal adhesions and abscesses were noted at 7 day after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), tissue-type plasminogen activator (tPA), plasminogen activator inhibitor type 1 (PAI-1), and tumor necrosis factor (TNF)- alpha was performed to assess the cecal tissue.
Results:
Adhesion formation was significantly reduced in PG, PL, CMC, and HA groups (P < 0.001) compared with that in RL group. The incidence of abscesses was also significantly reduced in PG and PL groups (P < 0.05) compared with that in the RL group. The level of uPA, uPAR, tPA, and TNF-alpha was highly expressed in PG and PL group, as compared with the RL group.
Conclusions:
We concluded that PG and PL had significant adhesion- and abscess-reducing effects and may act by modulating fibrinolytic capacity of uPA and/or tPA produced from macrophages in a rat peritonitis model.
Insights
Fungal polysaccharides from Phellinus gilvus (PG) and Phellinus linteus (PL) significantly reduced adhesions and abscesses in a rat peritonitis model. These compounds may enhance the body's natural healing processes.
Area of Science:
- Biomedical Science
- Pharmacology
- Immunology
Background:
- Phellinus spp. polysaccharides are known for anti-inflammatory effects.
- Limited data exists on their efficacy in preventing intraperitoneal adhesions.
- This study investigates the anti-adhesion and anti-abscess properties of Phellinus gilvus (PG) and Phellinus linteus (PL) polysaccharides.
Purpose of the Study:
- To evaluate the potential of fungal polysaccharides (PG and PL) in reducing intraperitoneal adhesions and abscess formation.
- To assess the impact of these polysaccharides on key molecular markers in a rat peritonitis model.
Main Methods:
- A rat peritonitis model was established using cecal ligation and puncture (CLP) in 60 Sprague-Dawley rats.
- Animals were divided into five groups: Ringer's lactate (RL), PG, PL, hyaluronic acid (HA), and carboxymethylcellulose (CMC).
- Adhesions and abscesses were evaluated 7 days post-CLP, with RT-PCR used to analyze gene expression of urokinase-type plasminogen activator (uPA), uPAR, tPA, PAI-1, and TNF-alpha.
Main Results:
- PG, PL, CMC, and HA groups showed significant reduction in adhesion formation compared to the RL group (P < 0.001).
- Abscess incidence was significantly lower in the PG and PL groups versus the RL group (P < 0.05).
- Expression of uPA, uPAR, tPA, and TNF-alpha was notably higher in the PG and PL groups compared to the RL group.
Conclusions:
- Polysaccharides from Phellinus gilvus (PG) and Phellinus linteus (PL) demonstrate significant efficacy in reducing adhesions and abscesses.
- These effects are potentially mediated by modulating the fibrinolytic system through urokinase-type plasminogen activator (uPA) and/or tissue-type plasminogen activator (tPA) in macrophages.
