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Updated: Aug 15, 2026
12:45
Preparation of Viral DNA from Nucleocapsids
Published on: August 16, 2011
A rapid method for purification of herpesvirus DNA
1State Veterinary Institute for Virus Research, Lindholm, Kalvehave, Denmark.
Journal of Virological Methods
|April 1, 1992
Abstract:
A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.
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