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Staphylococcus aureus ampicillin-resistant from the odontological clinic environment
Wagner Luis de Carvalho Bernardo1, Marcelo Fabiano Gomes Boriollo, Reginaldo Bruno Gonçalves
1Departamento de Diagnóstico Oral, Laboratório de Microbiologia e Imunologia, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, Piracicaba, SP, Brazil. pgmicro@fop.unicamp.br
Revista Do Instituto De Medicina Tropical De Sao Paulo
|February 25, 2005
Summary
Staphylococcus aureus was found in dental clinic air, with some strains producing beta-lactamase and showing antibiotic resistance. Further research is needed to understand contamination and spread in dental settings.
Area of Science:
- Microbiology
- Environmental Health
- Dental Public Health
Background:
- Staphylococcus species are common in healthcare environments.
- Odontological clinics can harbor airborne microorganisms.
- Understanding microbial contamination is crucial for infection control.
Purpose of the Study:
- To determine the prevalence of Staphylococcus species and S. aureus in dental clinic air.
- To assess beta-lactamase production and antibiotic susceptibility of isolated strains.
- To investigate potential contamination sources and propagation in dental settings.
Main Methods:
- Air sampling over 12 months in an odontological clinic.
- Isolation and identification of Staphylococcus spp. and S. aureus using MSA and chromogenic media.
- Gram staining, catalase, and coagulase tests for bacterial identification.
- Beta-lactamase production testing with Cefinase discs.
- Antibiotic susceptibility testing and oxacillin Minimum Inhibitory Concentration (MIC) determination via Etest.
Main Results:
- High levels of Staphylococcus spp. (9775 CFU) detected in clinic air, potentially via aerosols.
- 32.2% of isolates were suggestive of pathogenic staphylococci.
- 44 S. aureus isolates (0.45%) were confirmed.
- 79.5% of S. aureus produced beta-lactamase and exhibited resistance to ampicillin, erythromycin, and tetracycline.
- Oxacillin MIC results suggested the absence of the mecA gene.
Conclusions:
- Dental clinic air harbors Staphylococcus species, including S. aureus.
- Isolated S. aureus strains demonstrate beta-lactamase production and multi-drug resistance.
- Further investigation into contamination pathways and microbial spread within dental environments is warranted.