Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SNP genotyping using microsphere-linked PNA and flow cytometric detection.

Eszter Rockenbauer1, Kenneth Petersen, Ulla Vogel

  • 1Institute for Human Genetics, University of Aarhus, Aarhus, Denmark.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|February 25, 2005
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reduced eccDNA content in idiopathic asthenozoospermia sperm is associated with compromised DNA repair capacity and elevated DNA damage.

Cellular & molecular biology letters·2026
Same author

Energy metabolism alteration and gene expression reprogramming in a cell model of high fat load non-alcoholic fatty liver disease.

BMC gastroenterology·2026
Same author

Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and the Tumor Immune Microenvironment.

Cancer discovery·2025
Same author

An Overview and Comparative Analysis of CRISPR-SpCas9 gRNA Activity Prediction Tools.

The CRISPR journal·2025
Same author

Spatial-Temporal Diversity of Extrachromosomal DNA Shapes Urothelial Carcinoma Evolution and Tumor-Immune Microenvironment.

Cancer discovery·2025
Same author

Mapping the chromothripsis landscape in urothelial carcinoma unravels great intratumoral and intertumoral heterogeneity.

iScience·2025

This study introduces a novel method for single nucleotide polymorphism (SNP) genotyping using peptide nucleic acid (PNA) technology and flow cytometry. The PNA-based assay provides a fast, accurate, and robust approach for genetic variation analysis.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Single nucleotide polymorphisms (SNPs) are common genetic variations.
  • Peptide nucleic acids (PNAs) offer sensitive SNP genotyping methods.
  • A new assay combines PNA technology with flow cytometry for SNP detection.

Purpose of the Study:

  • To develop and validate a novel SNP genotyping method.
  • To assess the efficacy of PNA-based allele-specific hybridization.
  • To evaluate flow cytometric detection for genetic analysis.

Main Methods:

  • Genomic DNA amplification using two-step polymerase chain reaction (PCR).
  • Coupling of allele-specific PNA molecules to microspheres.
  • Allele-specific hybridization and flow cytometric detection.

Related Experiment Videos

Main Results:

  • A functional two-bead genotyping system was developed.
  • The system accurately and rapidly genotyped a basal cell carcinoma cohort.
  • Successful application of PNA capture and flow cytometry for SNP analysis.

Conclusions:

  • The new assay is simple, rapid, and robust for SNP genotyping.
  • It is suitable for laboratories with standard flow cytometers.
  • The system has potential for multiplexing and middle-scale genotyping.