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Updated: Aug 19, 2026
Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
Published on: May 18, 2018
[Study on the anti-maturation features of immature dendritic cells induced by low dose of granulocyte macrophage
1Institute of Burn Research, Southwest Hospital, State Key Laboratory of Trauma, Burns and Combined Injury, The Third Military Medical University, Chongqing, 400038, P.R. China.
Objective:
To investigate the influence of maturative agents, including lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF-alpha) and interferon gamma(IFN-gamma) on the maturation of immature dendritic cells originated from murine bone marrow induced by low dose of granulocyte macrophage colony stimulating factor (rm GM-CSF).
Methods:
Dendritic cells from murine bone marrow progenitors were cultured in low and high doses of GM-CSF for 6 days, and then the suspending cells were harvested for the experiment. After 3 days of co-culture of the obtained DC with low dose rmGM-CSF (GM(low)DC) with LPS, TNF-alpha and IFN-gamma, the stimulatory capacity of inducing proliferation of non-sensitized splenocytes of GM(low)DC in mixed lymphocyte reaction (MLR) was observed and compared with that of GM(high)DC.
Results:
GM(low)DC could not activate the non-sensitized splenocytes or induce it into proliferation after 3 days of co-incubation with LPS, TNF-alpha, IFN-gamma, with the stimulation index (SI) lower than 2. Whereas GM(high)DC could strongly activate naive splenocytes (SI = 4.71).
Conclusion:
GM(low)DC was resistant to maturation and insensitive to the stimulation by LPS, TNF-alpha or IFN-gamma.
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