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Macrophage inflammatory protein 1 modulates macrophage function.

T J Fahey1, K J Tracey, P Tekamp-Olson

  • 1Picower Institute for Medical Research, Manhasset, NY 11030.

Journal of Immunology (Baltimore, Md. : 1950)
|May 1, 1992
PubMed
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Macrophage inflammatory protein 1 (MIP 1) peptides modulate macrophage function, enhancing tumor cell killing and cytokine secretion. MIP 1 alpha activates macrophages, while MIP 1 beta can inhibit this activation, suggesting autocrine roles in inflammation.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Macrophage inflammatory protein 1 (MIP 1) is a heparin-binding protein doublet.
  • MIP 1 exhibits pyrogenic, mitogenic, and proinflammatory effects on various cell types.
  • The specific actions of MIP 1 on macrophages, its cell of origin, are not well-documented.

Purpose of the Study:

  • To investigate the effects of MIP 1 on macrophage functions.
  • To determine the distinct roles of MIP 1 alpha and MIP 1 beta peptides.
  • To explore the potential autocrine modulatory role of MIP 1 in macrophages.

Main Methods:

  • Treatment of macrophages (WEHI tumor cells, peritoneal exudate macrophages) with MIP 1, MIP 1 alpha, and MIP 1 beta.
  • Assays for macrophage cytotoxicity, proliferation, cytokine secretion (TNF, IL-6, IL-1 alpha), oxidative burst, and Ia expression.

Related Experiment Videos

  • Co-stimulation experiments with CSF-1, granulocyte-macrophage-CSF, and IFN-gamma.

    • Main Results:

    • MIP 1 enhanced antibody-independent macrophage cytotoxicity against tumor targets.
    • MIP 1 stimulated macrophage proliferation, an effect boosted by CSF-1 or granulocyte-macrophage-CSF.
    • MIP 1 alpha induced secretion of TNF, IL-1 alpha, and IL-6; MIP 1 beta inhibited MIP 1 alpha-induced TNF secretion.
    • MIP 1 did not trigger the oxidative burst or up-regulate Ia expression.

    Conclusions:

    • MIP 1 peptides act as autocrine modulators of macrophage function.
    • MIP 1 alpha is an activator of macrophages, while MIP 1 beta can act as an inhibitor.
    • MIP 1 may play a role in regulating macrophage responses during inflammation in vivo.